Measurement

Part:BBa_J70605:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-06-28)


J70590 cut with BsaXI and with GSGSGS inserts (J70556-fi,ri) added


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 854


Design Notes

Created by cutting part J70590 with BsaXI and adding glycine-serine-glycine-serine-glycine serine inserts (legacy from prior design)

forward fusion (J70556-fi): aaggatctggatctggatctaAGA

reverse fusion (J70556-ric): TAGATCCAGATCCAGATCCTTGTT


Source

Look at part J70575

References